All-Natural Gelatin-Based Nanoemulsion Loaded with TLR 7/8 Agonist for Efficient Modulation of Macrophage Polarization for Immunotherapy

All-Natural Gelatin-Based Nanoemulsion Loaded with TLR 7/8 Agonist for Efficient Modulation of Macrophage Polarization for Immunotherapy

Blog Article

Macrophages are multifunctional immune cells essential for both innate and adaptive immune responses.Tumor-associated macrophages (TAMs) often adopt a tumor-promoting Microwave Door Frame M2-like phenotype, aiding tumor progression and immune evasion.Reprogramming TAMs to a tumoricidal M1-like phenotype is an emerging target for cancer immunotherapy.Resiquimod, a TLR7/8 agonist, can repolarize macrophages from the M2- to M1-like phenotype but is limited by poor Microwaves solubility.

We developed a gelatin nanoemulsion for the loading and delivery of resiquimod, utilizing eugenol oil as the liquid phase and riboflavin-crosslinked gelatin as a scaffold.These nanoemulsions showed high stability, low toxicity, and effective macrophage repolarization, significantly enhancing pro-inflammatory markers and anticancer activity in co-culture models.